Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids.
Identifieur interne : 000365 ( Main/Exploration ); précédent : 000364; suivant : 000366Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids.
Auteurs : Chengjuan Zou [République populaire de Chine] ; Pan Wang [République populaire de Chine] ; Shuli Liang [République populaire de Chine] ; Ying Lin [République populaire de Chine]Source :
- Biotechnology letters [ 1573-6776 ] ; 2019.
Descripteurs français
- KwdFr :
- Acides aminés (métabolisme), Analyse de profil d'expression de gènes (MeSH), Autophagie (MeSH), Complexe-1 cible mécanistique de la rapamycine (métabolisme), Délétion de séquence (MeSH), Milieux de culture (composition chimique), Méthanol (métabolisme), Pichia (croissance et développement), Pichia (génétique), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Systèmes de transport d'acides aminés (métabolisme).
- MESH :
- composition chimique : Milieux de culture.
- croissance et développement : Pichia.
- génétique : Pichia, Protéines fongiques.
- métabolisme : Acides aminés, Complexe-1 cible mécanistique de la rapamycine, Méthanol, Protéines fongiques, Systèmes de transport d'acides aminés.
- Analyse de profil d'expression de gènes, Autophagie, Délétion de séquence.
English descriptors
- KwdEn :
- Amino Acid Transport Systems (metabolism), Amino Acids (metabolism), Autophagy (MeSH), Culture Media (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Profiling (MeSH), Mechanistic Target of Rapamycin Complex 1 (metabolism), Methanol (metabolism), Pichia (genetics), Pichia (growth & development), Sequence Deletion (MeSH).
- MESH :
- chemical , chemistry : Culture Media.
- chemical , genetics : Fungal Proteins.
- chemical , metabolism : Amino Acid Transport Systems, Amino Acids, Fungal Proteins, Mechanistic Target of Rapamycin Complex 1, Methanol.
- genetics : Pichia.
- growth & development : Pichia.
- Autophagy, Gene Expression Profiling, Sequence Deletion.
Abstract
OBJECTIVE
The purpose of this article is to study the underlying cause of the induction of autophagy in Pichia pastoris cells grown in amino acid-rich methanol medium during methanol adaptation.
RESULTS
Autophagy was induced in P. pastoris GS115 when cells were grown in amino acid-rich methanol medium. Transcriptome analysis revealed that genes involved in amino acid biosynthesis were upregulated. The deletion of Gcw13, a GPI-anchored protein that plays a role in the endocytosis of the general amino acid permease Gap1, resulted in the inhibition of autophagy, the activation of TORC1 and an increase in the uptake of glutamine and asparagine in methanol-grown cells.
CONCLUSIONS
Our results demonstrated that the autophagy induced in P. pastoris cells grown in amino acid-rich methanol medium was nitrogen source independent and may be due to a Gcw13-dependent decrease in amino acid uptake during methanol adaptation.
DOI: 10.1007/s10529-019-02744-9
PubMed: 31650421
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids.</title><author><name sortKey="Zou, Chengjuan" sort="Zou, Chengjuan" uniqKey="Zou C" first="Chengjuan" last="Zou">Chengjuan Zou</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Pan" sort="Wang, Pan" uniqKey="Wang P" first="Pan" last="Wang">Pan Wang</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Shuli" sort="Liang, Shuli" uniqKey="Liang S" first="Shuli" last="Liang">Shuli Liang</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China. feylin@scut.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. feylin@scut.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. feylin@scut.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31650421</idno><idno type="pmid">31650421</idno><idno type="doi">10.1007/s10529-019-02744-9</idno><idno type="wicri:Area/Main/Corpus">000168</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000168</idno><idno type="wicri:Area/Main/Curation">000168</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000168</idno><idno type="wicri:Area/Main/Exploration">000168</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids.</title><author><name sortKey="Zou, Chengjuan" sort="Zou, Chengjuan" uniqKey="Zou C" first="Chengjuan" last="Zou">Chengjuan Zou</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Pan" sort="Wang, Pan" uniqKey="Wang P" first="Pan" last="Wang">Pan Wang</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Shuli" sort="Liang, Shuli" uniqKey="Liang S" first="Shuli" last="Liang">Shuli Liang</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><affiliation wicri:level="3"><nlm:affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China. feylin@scut.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. feylin@scut.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. feylin@scut.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006</wicri:regionArea><wicri:noRegion>510006</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biotechnology letters</title><idno type="eISSN">1573-6776</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Transport Systems (metabolism)</term><term>Amino Acids (metabolism)</term><term>Autophagy (MeSH)</term><term>Culture Media (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Mechanistic Target of Rapamycin Complex 1 (metabolism)</term><term>Methanol (metabolism)</term><term>Pichia (genetics)</term><term>Pichia (growth & development)</term><term>Sequence Deletion (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (métabolisme)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Autophagie (MeSH)</term><term>Complexe-1 cible mécanistique de la rapamycine (métabolisme)</term><term>Délétion de séquence (MeSH)</term><term>Milieux de culture (composition chimique)</term><term>Méthanol (métabolisme)</term><term>Pichia (croissance et développement)</term><term>Pichia (génétique)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Systèmes de transport d'acides aminés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acid Transport Systems</term><term>Amino Acids</term><term>Fungal Proteins</term><term>Mechanistic Target of Rapamycin Complex 1</term><term>Methanol</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Pichia</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides aminés</term><term>Complexe-1 cible mécanistique de la rapamycine</term><term>Méthanol</term><term>Protéines fongiques</term><term>Systèmes de transport d'acides aminés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Autophagy</term><term>Gene Expression Profiling</term><term>Sequence Deletion</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Autophagie</term><term>Délétion de séquence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>The purpose of this article is to study the underlying cause of the induction of autophagy in Pichia pastoris cells grown in amino acid-rich methanol medium during methanol adaptation.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Autophagy was induced in P. pastoris GS115 when cells were grown in amino acid-rich methanol medium. Transcriptome analysis revealed that genes involved in amino acid biosynthesis were upregulated. The deletion of Gcw13, a GPI-anchored protein that plays a role in the endocytosis of the general amino acid permease Gap1, resulted in the inhibition of autophagy, the activation of TORC1 and an increase in the uptake of glutamine and asparagine in methanol-grown cells.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Our results demonstrated that the autophagy induced in P. pastoris cells grown in amino acid-rich methanol medium was nitrogen source independent and may be due to a Gcw13-dependent decrease in amino acid uptake during methanol adaptation.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31650421</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN><JournalIssue CitedMedium="Internet"><Volume>41</Volume><Issue>12</Issue><PubDate><Year>2019</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids.</ArticleTitle><Pagination><MedlinePgn>1423-1431</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-019-02744-9</ELocationID><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">The purpose of this article is to study the underlying cause of the induction of autophagy in Pichia pastoris cells grown in amino acid-rich methanol medium during methanol adaptation.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Autophagy was induced in P. pastoris GS115 when cells were grown in amino acid-rich methanol medium. Transcriptome analysis revealed that genes involved in amino acid biosynthesis were upregulated. The deletion of Gcw13, a GPI-anchored protein that plays a role in the endocytosis of the general amino acid permease Gap1, resulted in the inhibition of autophagy, the activation of TORC1 and an increase in the uptake of glutamine and asparagine in methanol-grown cells.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Our results demonstrated that the autophagy induced in P. pastoris cells grown in amino acid-rich methanol medium was nitrogen source independent and may be due to a Gcw13-dependent decrease in amino acid uptake during methanol adaptation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zou</LastName><ForeName>Chengjuan</ForeName><Initials>C</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-5583-2785</Identifier><AffiliationInfo><Affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Pan</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Shuli</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Biology and Biological Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China. feylin@scut.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. feylin@scut.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. feylin@scut.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>31170031</GrantID><Agency>the National Natural Science Foundation of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026905">Amino Acid Transport Systems</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber><NameOfSubstance UI="D000076222">Mechanistic Target of Rapamycin Complex 1</NameOfSubstance></Chemical><Chemical><RegistryNumber>Y4S76JWI15</RegistryNumber><NameOfSubstance UI="D000432">Methanol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D026905" MajorTopicYN="N">Amino Acid Transport Systems</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000596" MajorTopicYN="N">Amino Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001343" MajorTopicYN="Y">Autophagy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000076222" MajorTopicYN="N">Mechanistic Target of Rapamycin Complex 1</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000432" MajorTopicYN="N">Methanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010843" MajorTopicYN="N">Pichia</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017384" MajorTopicYN="Y">Sequence Deletion</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Amino acid starvation</Keyword><Keyword MajorTopicYN="N">Autophagy</Keyword><Keyword MajorTopicYN="N">Gcw13</Keyword><Keyword MajorTopicYN="N">Pichia pstoris</Keyword><Keyword MajorTopicYN="N">TORC1</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>10</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31650421</ArticleId><ArticleId IdType="doi">10.1007/s10529-019-02744-9</ArticleId><ArticleId IdType="pii">10.1007/s10529-019-02744-9</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Zou, Chengjuan" sort="Zou, Chengjuan" uniqKey="Zou C" first="Chengjuan" last="Zou">Chengjuan Zou</name></region><name sortKey="Liang, Shuli" sort="Liang, Shuli" uniqKey="Liang S" first="Shuli" last="Liang">Shuli Liang</name><name sortKey="Liang, Shuli" sort="Liang, Shuli" uniqKey="Liang S" first="Shuli" last="Liang">Shuli Liang</name><name sortKey="Liang, Shuli" sort="Liang, Shuli" uniqKey="Liang S" first="Shuli" last="Liang">Shuli Liang</name><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><name sortKey="Lin, Ying" sort="Lin, Ying" uniqKey="Lin Y" first="Ying" last="Lin">Ying Lin</name><name sortKey="Wang, Pan" sort="Wang, Pan" uniqKey="Wang P" first="Pan" last="Wang">Pan Wang</name><name sortKey="Wang, Pan" sort="Wang, Pan" uniqKey="Wang P" first="Pan" last="Wang">Pan Wang</name><name sortKey="Wang, Pan" sort="Wang, Pan" uniqKey="Wang P" first="Pan" last="Wang">Pan Wang</name><name sortKey="Zou, Chengjuan" sort="Zou, Chengjuan" uniqKey="Zou C" first="Chengjuan" last="Zou">Chengjuan Zou</name><name sortKey="Zou, Chengjuan" sort="Zou, Chengjuan" uniqKey="Zou C" first="Chengjuan" last="Zou">Chengjuan Zou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000365 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000365 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31650421
|texte= Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31650421" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |